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custom de novo gene synthesis  (Twist Bioscience)
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(a) Time-series OD600 measurements acquired every 20 min for 72 h at 37°C. Points denote the mean of three technical replicates; grey error bars indicate SD. Rows correspond to successive 10-fold dilutions. Conditions: wild-type T7 (positive control, PC), AMP-loaded POPs (AMP), POPs, and HPLC-grade water (negative control, NC). (b) Dose-effect relationship of wild-type T7 phages and engineered POPs based on antimicrobial susceptibility testing (AST) assays, shown as the relative increase in OD600 at 12 hours compared to the initial OD600. (c) Workflow for POP generation and testing: 1. Design and de <t>novo</t> synthesize modular linear <t>gene</t> fragments encoding antimicrobial and structural proteins. 2: Reboot fragments in a cell-free protein <t>synthesis</t> system to generate POPs. 3: <t>Culture</t> the host bacteria in LB broth at 37°C, 220 rpm overnight. 4: Dispense cells into 96-well plates. 5: Apply serial dilutions of POPs across the plate. 6: assess inhibitory activity by time-series monitoring of OD600.
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custom gene synthesis  (General Biosystems Inc)
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(a) Time-series OD600 measurements acquired every 20 min for 72 h at 37°C. Points denote the mean of three technical replicates; grey error bars indicate SD. Rows correspond to successive 10-fold dilutions. Conditions: wild-type T7 (positive control, PC), AMP-loaded POPs (AMP), POPs, and HPLC-grade water (negative control, NC). (b) Dose-effect relationship of wild-type T7 phages and engineered POPs based on antimicrobial susceptibility testing (AST) assays, shown as the relative increase in OD600 at 12 hours compared to the initial OD600. (c) Workflow for POP generation and testing: 1. Design and de <t>novo</t> synthesize modular linear <t>gene</t> fragments encoding antimicrobial and structural proteins. 2: Reboot fragments in a cell-free protein <t>synthesis</t> system to generate POPs. 3: <t>Culture</t> the host bacteria in LB broth at 37°C, 220 rpm overnight. 4: Dispense cells into 96-well plates. 5: Apply serial dilutions of POPs across the plate. 6: assess inhibitory activity by time-series monitoring of OD600.
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gene forge® codon optimization and custom gene synthesis platform  (Aptagen Inc)
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(a) Time-series OD600 measurements acquired every 20 min for 72 h at 37°C. Points denote the mean of three technical replicates; grey error bars indicate SD. Rows correspond to successive 10-fold dilutions. Conditions: wild-type T7 (positive control, PC), AMP-loaded POPs (AMP), POPs, and HPLC-grade water (negative control, NC). (b) Dose-effect relationship of wild-type T7 phages and engineered POPs based on antimicrobial susceptibility testing (AST) assays, shown as the relative increase in OD600 at 12 hours compared to the initial OD600. (c) Workflow for POP generation and testing: 1. Design and de <t>novo</t> synthesize modular linear <t>gene</t> fragments encoding antimicrobial and structural proteins. 2: Reboot fragments in a cell-free protein <t>synthesis</t> system to generate POPs. 3: <t>Culture</t> the host bacteria in LB broth at 37°C, 220 rpm overnight. 4: Dispense cells into 96-well plates. 5: Apply serial dilutions of POPs across the plate. 6: assess inhibitory activity by time-series monitoring of OD600.
Gene Forge® Codon Optimization And Custom Gene Synthesis Platform, supplied by Aptagen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gene forge® codon optimization and custom gene synthesis platform - by Bioz Stars, 2026-07
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(a) Time-series OD600 measurements acquired every 20 min for 72 h at 37°C. Points denote the mean of three technical replicates; grey error bars indicate SD. Rows correspond to successive 10-fold dilutions. Conditions: wild-type T7 (positive control, PC), AMP-loaded POPs (AMP), POPs, and HPLC-grade water (negative control, NC). (b) Dose-effect relationship of wild-type T7 phages and engineered POPs based on antimicrobial susceptibility testing (AST) assays, shown as the relative increase in OD600 at 12 hours compared to the initial OD600. (c) Workflow for POP generation and testing: 1. Design and de novo synthesize modular linear gene fragments encoding antimicrobial and structural proteins. 2: Reboot fragments in a cell-free protein synthesis system to generate POPs. 3: Culture the host bacteria in LB broth at 37°C, 220 rpm overnight. 4: Dispense cells into 96-well plates. 5: Apply serial dilutions of POPs across the plate. 6: assess inhibitory activity by time-series monitoring of OD600.

Journal: bioRxiv

Article Title: Targeting multidrug-resistant bacteria with genetic-information-free protein-only phages

doi: 10.1101/2025.10.13.682247

Figure Lengend Snippet: (a) Time-series OD600 measurements acquired every 20 min for 72 h at 37°C. Points denote the mean of three technical replicates; grey error bars indicate SD. Rows correspond to successive 10-fold dilutions. Conditions: wild-type T7 (positive control, PC), AMP-loaded POPs (AMP), POPs, and HPLC-grade water (negative control, NC). (b) Dose-effect relationship of wild-type T7 phages and engineered POPs based on antimicrobial susceptibility testing (AST) assays, shown as the relative increase in OD600 at 12 hours compared to the initial OD600. (c) Workflow for POP generation and testing: 1. Design and de novo synthesize modular linear gene fragments encoding antimicrobial and structural proteins. 2: Reboot fragments in a cell-free protein synthesis system to generate POPs. 3: Culture the host bacteria in LB broth at 37°C, 220 rpm overnight. 4: Dispense cells into 96-well plates. 5: Apply serial dilutions of POPs across the plate. 6: assess inhibitory activity by time-series monitoring of OD600.

Article Snippet: Custom de novo gene synthesis was performed according to the provider’s proprietary protocols (Twist Bioscience, USA) via high-throughput, silicon-based synthesis per provider protocols with a reported average error rate of 0.013%.

Techniques: Positive Control, Negative Control, Bacteria, Activity Assay